Forms
Download and fill out the forms below. If you have questions, please contact Anita Saraf, the director of MSAPL.
Instructions
- Contact us with questions about your project/samples at any time
- Fill out the request form(s) below and discuss your project before sending samples
- Provide us with as much information about your sample as you can
- Send the protein sequence (FASTA format) when you have any tags attached to the original sequence or sequence is mutated
- Wear gloves at all times during sample preparation
- Avoid contaminants
- Avoid viscous material, polymers, particulates and detergents
- Label samples clearly
- Avoid glycerol/sucrose in your samples, dialyze it out or keep it as low as possible as it will clog the nano-LC column
- Avoid high detergents (e.g., >2%SDS), DNA/RNA in your samples
- Avoid protein contaminants, such as BSA, Keratins, large protein tags in your samples
- Email or return completed forms to Anita Saraf, director of MSAPL
How to best prepare samples to be analyzed by shotgub proteomics approach: Depending on the sample complexity, we require 1-2ug starting material for protein-IDs. Post-translational modifications (PTMs) analysis requires three times that amount to allow for multiple enzyme digestions to be used to obtain comprehensive sequence coverage of protein/s of interest.
You will receive results in the form of an Excel spreadsheet detailing all the detected proteins and other protein related information. The file will be custom-made specific to project goals and the results we generate. You will also receive a detailed report as a .pdf file.
We will provide updated rates soon.